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Weight loss, improved glycaemic control, and reduced insulin resistance all contribute to decreased systemic inflammation

Raw reads were mapped to the GENCODE vM9 mouse reference genome (GENCODE) using STAR Aligner with the following options: outFilterMultimapNmax 15 outFilterMismatchNmax 6 outSAMstrandField All outSAMtype BAM SortedByCoordinate quantMode TranscriptomeSAM

Cytoplasmic free Ca 2+ concentration imaging For live-cell cytoplasmic free Ca 2+ concentration ([Ca 2+ ] i ) imaging experiments, Gcg-GCaMP6f islets were immobilised to a poly-l-lysine-coated coverslip fixed in a custom-built imaging chamber filled with EC3 (ESM Table 2)

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Genes Dev 13 : 2634