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Description

Excess DTT and imidazole were removed on a PD-10 desalting column (Merck), and the reduced proteins were eluted with 3.5 mL ice-cold assay buffer containing 100 mM Tris/HCl, 1 mM EDTA, pH 8.0

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USA 109 , 10921097 (2012)

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Studies performed so far regarding the use of antioxidant agents, especially as selenium, provided conflicting results on the possibility of a new therapeutic approach

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