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"It's really important that scientific discoveries are translated from the bench cells, animals into humans through a series of clinical trials that prove at every step, with increasing numbers of subjects, that this product is effective, that it's safe, and that you know how much of it to administer." With files from Ariel Tozman

Point mutations and NNK site saturation libraries of PtGSTF8 were constructed by overlap extension PCR as previously reported 49 using primers T7 fw, T7 term and: PtGSTF8 (V12X): V12C13_rv and V12X_fw, PtGSTF8 (C13X): V12C13_rv and C13X_fw, PtGSTF8 (C13S): V12C13_rv and C13S_fw, PtGSTF8 (N108X): N108_rv, N108X_fw, PtGSTF8 (F112X): F112_rv, F112X_fw (Supplementary Table 4) using pANT35 as template

Storage conditions, handling technique, and date tracking all determine whether a vial stays safe through that full window

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