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For 10 days, healthy subjects received 10 mg of oral semaglutide (formulation R1) once daily doses with 50 mL or 120 mL of water under fasting conditions with post-dose fasting period of 15, 30, 60 or 120 minutes

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3.1.3 DON DON (6-diazo-5-oxo-l-norleucine), a glutamine analog, irreversibly inhibits various glutamine utilization enzymes, such as glutaminase and glutamine synthetase, by forming covalent bonds with nucleophilic residues on the active site of the enzyme (Rajagopalan and DeBerardinis, 2011

Primers used to construct deletion mutants are listed in Table S2