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At each time point (five minutes intervals), photoluminescence spectra was recorded using a FluoroMax 4 (Horiba, Jobin Yvon GmbH, Unterhaching, Germany) spectrophotometer working with a continuous 450 W xenon lamp at ex / em of: 365/605, 552/636 and 495/519 nm for PLGA encapsulating DDA-GSH-AuNCs, Nile red or fluorescein, respectively

After centrifugation, spermatozoa suspension was sonicated for 10 s on ice repeating six times at intervals of 30 s (66)

It is also produced in the USA from global ingredients and 3rd party lab tested for purity and potency

Root causes of glutathione deficiency: 1

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