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It activates several overlapping pathways, notably VEGFR2 and nitric oxide synthesis via the Akt-eNOS axis, promoting angiogenesis, fibroblast activity, and neuromuscular stabilization

DTNB reacts with GSH to form a yellow product that can be measured spectrophotometrically at an absorbance of 412 nm

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It is a coordination complex: a tripeptide ligand, glycyl-histidyl-lysine, bound to copper in the divalent state through the N-terminal amine, a deprotonated backbone amide nitrogen and the imidazole nitrogen of the histidine side chain