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This protocol consists of infusing half the 36 mM NAC dose over a 1-hour period starting at 2 hours after the APAP dose, followed by 1/6 of the dose over the next 4-hour period and then 1/3 of the dose over an additional 16 hours
The pulsatile nature of GH release induced by this combination, rather than continuous elevation, has been identified as a key factor in maintaining receptor sensitivity and avoiding desensitization artifacts that can complicate long-term research protocols
