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Strategies for Monitoring and Supplementation Given the consistent evidence, anyone on a GLP-1 medication should treat vitamin D monitoring as a non-negotiable part of their treatment protocol[4]Vitamin D Fact Sheet NIH ODS View source: Baseline and follow-up serum testing check 25(OH)D levels before starting GLP-1 therapy and every 6 months thereafter.[10]Vitamin D Deficiency N Engl J Med View source Target range: 4060 ng/mL Vitamin D3 supplementation 2,0004,000 IU/day of vitamin D3 (see full dosage guide), adjusted based on blood test results.[14]Vitamin D Receptor in Nerve Cells Journal of Steroid Biochemistry View source Take with the fattiest meal of the day for optimal absorption[5]Vitamin D Absorption with Fat NCBI View source Pair with calcium and magnesium vitamin D works synergistically with both