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Glutathione neutralizes free radicals
The mushrooms are often dried, ground, and processed to concentrate their bioactive compounds
Arch Pharm Res, 2005
After centrifugation, 10 l of the supernatant were filtered through a Microcon YM-10 centrifugal filter device (Amicon), and analyzed by reversed-phase high-performance liquid chromatography on a C18 column (Atlantis 5 m, 4,6200 mm, Waters) as previously described [18]
These included two substitutions impacting key structural residues or nearby residues (P134L and S229T), and three involving residues relatively distant from known essential elements (D176N, R215H, and P330L)
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