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Briefly, neuronal homogenates extracts were diluted in Potassium phosphate-EDTA (KPE) buffer such that the sample values would fall within the standard curve range
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Since the latter study had a placebo group which had redox and methylation biomarkers measured at the beginning and end of the intervention, we use this placebo group as a control group for all three studies in order to determine if the changes in redox and methylation metabolism were significant as a result of the three interventions
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