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The researchers evaluated various oxidative stress markers, including protein carbonylation, protein-bound 4-hydroxy-2-nonenal (HNE), levels of total and oxidized glutathione (GSH), concentrations of heat shock protein (HSP) and thioredoxin (TRX), in amniotic fluid samples

Materals and methods Materials MTX was obtained from David Bull Laboratories, Mulgrave-Victoria, Australia

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Induction of Parkinson's Disease Parkinson's disease was induced by intraperitoneal injection (i.p.) of Rotenone at a dose of 2 mg/kg of body weight, every day for 21 days and was administered 1 h before standard drug or treatment [19]