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even if some statistical differences emerged, they may be considered negligible from the practical point of view, with values quite similar among all the strains (about 107 cells/mL), highlighting a good growing ability in the conditions tested

In this report we demonstrate the efficacy of multiplexing the CellTox Green Cytotoxicity Assay with the GSH/GSSG-Glo Assay to determine both the cytotoxic and oxidative effects of compounds on cultured cells in a single assay plate

Tissue sections were permeabilized with 0.5% Triton X-100 for 20 min and then blocked with 5% goat serum for 30 min at room temperature

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Blocks the NNMT enzyme that converts nicotinamide to N-methylnicotinamide, preserving nicotinamide for NAD+ synthesis and cellular energy pathways