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In: StatPearls [Internet]

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Elutions were pooled, and the GST tag was removed from FAT via Thrombin Cleavage Kit (Sigma) into 50 mM Tris pH 8.0, 200 mM CaCl 2

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Namely, it is always applied alone (Sikiric et al., 1993

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How to Store Peptides After Reconstitution Introduction Reconstituted peptides should be kept refrigerated at 2 to 8 degrees Celsius (36 to 46 degrees Fahrenheit), protected from light, and used within 14 to 30 days depending on the peptide and the diluent

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