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Samples were heated at 95 C for 5 min with 4x Laemmli buffer supplemented with -mercaptoethanol, resolved by SDS-PAGE, and transferred to PVDF membranes using the Bio-Rad Trans-Blot Turbo Transfer System according to the manufacturers instructions

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The unfolded protein response and cellular senescence

High vitamin B12 is very common among athletes that supplement with vitamin B12

We observed a significant reduction in the expression of pro-inflammatory cytokines and inhibition of matrix metalloproteinases (MMPs), with particular emphasis on MMP-9