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Metabolites were resolved across a 2.1 150 mm, 1.7 m Kinetex C18 column (Phenomenex) using a 5-min, reverse-phase gradient from a previously described method 19
The derivatives were resolved and detected using liquid chromatography/electron spray ionization/tandem mass spectrometry in multi-reaction monitoring mode
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Dimethyl sulfoxide (DMSO) was used as the solvent for each compound before dilution with the medium
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