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The high-MW fractions form a transient occlusive layer on the surface during recovery, holding water and reducing transepidermal water loss in the 24-to-48-hour post-procedure window where the barrier is briefly compromised

Kumar, P., Shenoi, R.A., Lai, B.F., Nguyen, M., Kizhakkedathu, J.N., and Straus, S.K., Biomacromolecules , 2015, vol

Treatment discontinuation due to side effects occurred in 4.3%, 7.1%, 6.2%, and 2.6% of participants in the 5, 10, 15 mg, and placebo groups, respectively [18]

There is no pharmacological reason to separate the timing beyond using separate injection sites