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For characterization of cARLA-treated hECs the cells were cultured in 50% PC-conditioned medium on glass bottom chamber slides (Nunc Lab-Tek, Thermo Fisher Scientific) coated with collagen IV and fibronectin at a seeding density of 5 10 4 cells/cm 2

Reported side effects include [1][29]: Flatulence Loose stools Skin rash (rare) Flushing (rare) These side effects have generally been mild and self-limiting

96 UdovicicM.SeverM.KavurL.LoncaricK.BarisicI.BalenovicD.et al (2021)

Rosacea often manifests in recurring episodes of flushing, acne-like bumps and pustules, and sometimes severe skin damage with thickening, swelling, and nodules

Running them together amplifies that signal