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The 20 mg dose served as a mid-to-high exposure level within these escalation protocols, supporting subsequent phase 3 PIONEER trial development focusing on fixed 3, 7, and 14 mg maintenance doses

Shortly afterwards, in October 1964, Harold Elrick and his colleagues at the University of Colorado Medical School, independently showed that oral doses of glucose produced significantly higher levels of plasma insulin than when given by intravenous injection (Elrick, Stimler, Arai)