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If the vial was at room temperature for 1 to 7 days: it is probably still usable, but subtract those days from the 21-day room temperature maximum
Fair-skinned individuals with low baseline melanin might notice more dramatic visible changes from small shifts in pigment, and their tan can persist differently compared to someone with darker baseline skin
References 1 FosterNCBeckRWMillerKMClementsMARickelsMRDiMeglioLAet al
PVDF membrane was methanol-activated and soaked together with blotting paper in transfer buffer (25 mM Tris base, 150 mM glycine, 10% (v/v) methanol)
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