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These changes collectively contribute to a dysfunctional NVU, facilitating BBB and BSCB breakdown, subsequently allowing potentially neurotoxic substances from the periphery to enter the CNS parenchyma, exacerbating neurodegeneration [217]
On ice, the cells were washed in ice-cold PBS and incubated with a primary antibody targeting the extracellular part of PDGFR, 5 g/ml goat-anti-PDGFR (AF385, RnD Systems) in PBS for 1 h
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