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The samples were subjected to click reaction by adding 5 l biotin azide (final: 100 M), 25 l BTTAA (final: 500 M), 10 l CuSO 4 (final: 1 mM) and 10 l NaAsc (final: 2 mM) to a total volume of 500 l and incubated for 1 h at r.t

The pH of the medium was monitored using the phenol red indicator and remained within the physiological range (7.27.4) throughout the 72-hour period, ensuring that nutrient depletion or acidification did not confound the observed metabolic effects

Lubin, J

Important Safety Information: Gastrointestinal side effects like nausea are very common but are usually transient and managed by slow dose titration

This can lead to both short-term complications and unknown long-term consequences