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In the use of pH-sensitive hydrazone linkers, controlling non-specific hydrolysis is central to reducing adverse effects

GSSG is capable of causing protein S -glutathionylation or the reversible formation of protein-mixed disulfides (protein-SSG)

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Notably, we previously showed that administering Tempol (a potent ROS scavenger) or genetically deleting Ncf1 (which impairs the respiratory burst) led to bacterial outgrowth in mice 7