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First, the behavioral assessment in the present study focused primarily on anxiety-like behavior evaluated using the EPM

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Pinch the skin to create a fold, insert the needle at a 90-degree angle for most individuals or a 45-degree angle for very lean individuals, inject slowly, and hold for five seconds after depressing the plunger before withdrawing the needle

Most participants hit their plateau between weeks 40 and 48, with the average being around week 43

Enteroendocrine cells (EECs), the microbiota, and metabolites produced by the microbiota constitute a complex signaling system [13] that involves the enteric plexus, CNS afferents and efferents, and the modulation of numerous brain nuclei like the nucleus tractus solitarius, hypothalamus, thalamus, and many others directly involved in the control of glucose and energy homeostasis [14]