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Studies using high-performance liquid chromatography (HPLC) confirm that MT-1 degradation accelerates sharply after day 28, with potency dropping to 7080% by day 35
All the NMR experiments were carried out at 600.13 MHz for 1H detection, on BRUKER AVANCE NEO 600 spectrometer equipped with a cryogenic NMR probe to enhance the sensitivity
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Importantly, they can also convert indigestible carbohydrates into short-chain fatty acids (SCFA), which in turn activate specific G protein-coupled fatty acid (FFA) receptors, thereby promoting the secretion of peptide hormones from the enteroendocrine cells [106, 107]
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