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The total number of colonies found is multiplied by the dilution used, so the final calculation estimates the total concentration of bacteria in the original water sample

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CrossRef Coordination Among Lipid Droplets, Peroxisomes, and Mitochondria Regulates Energy Expenditure Through the CIDE-ATGL-PPAR Pathway in Adipocytes Linkang Zhou, Miao Yu, Muhammad Arshad, Wenmin Wang, Ye Lu, Jingyi Gong, Yangnan Gu, Peng Li, Li Xu Diabetes.2018

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A small percentage report slight bloating or water retention initially, which resolves within days