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Mass spectrometry The conversion of GSH into GSSG was monitored by mass spectrometry (Agilent 1290 UPLC/6540 Q-TOF)

Briefly, a 1 ml reaction mix (500 nM TGR, 100 M NADPH, and 2050 M inhibitor) was incubated at room temperature for 30 min for fast inhibitors, or 2 h for slow inhibitors

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The study demonstrated that Fer-1 effectively reversed the expression levels of the proteins SLC7A11, GPX4, and Tfr1 in SW480 cells (Fig