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After blocking with 5% bovine serum albumin (BSA, Sigma-Aldrich) in TBST (10 mM Tris-HCl, 150 mM NaCl, and 0.1% Tween 20) for 1 h at room temperature, the membrane was incubated with primary antibody overnight at 4 C

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A thiol bond is formed between the glutathione and an alipathic spacer that is linked to a hydroxyl group on the surface of the agarose bead [7]

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Pairing a GHRH analog with a ghrelin mimetic is a recurring theme in the literature, and it is also why blended research compounds exist, such as the tesamorelin and ipamorelin blend studied as a single preparation