The use of mediators provides redox connectivity between the melanin sample and electrode and this overcomes the major limitation of electrochemical analysis of insoluble samples 22
Among the main inflammatory mediators, CRP plays a crucial role in atherogenesis by promoting complement activation, enhancing macrophage lipid uptake, stimulating pro-inflammatory cytokine release, inducing monocyte tissue factor expression, impairing endothelial function, and inhibiting nitric oxide production, further aggravating vascular injury (Shrivastava et al
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