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Front In Endocrinol

For Lumi4-Tb, cells were labeled at 40 nM at 4 C, followed by time-resolved (TR) fluorescence measurement in a Molecular Devices i3x (excitation 335 nm, emission 620 nm)

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Protease activity was quenched by acidification with trifluoroacetic acid (TFA) to a final concentration of approximately 1% and the resulting peptide mixture was concentrated using reversed-phase Sep-Pak C18 cartridge (Waters)