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To stop the reaction, stop buffer (340 mM NaCl, 20 mM EDTA, 4 mM EGTA, 50 g/mL RNase A, 100 g/mL glycogen, and 3 pg/mL Drosophila DNA) was added, followed by incubation for 3 min at 4 C and for 39 min at 37 C

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At the end of the permeability assay midbrain organoids were removed from the bottom of acceptor compartments, homogenized in DW containing Triton X-100 (10 mg/ml)

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