RNeasy Mini Kit and QuantiFast SYBR Green PCR kit were purchased from Qiagen (Germany)
Unlike MT-1 10mg (Afamelanotide), which is selective for MC1R, MT-2 is a non-selective melanocortin agonist activating four receptor subtypes simultaneously: MC1R (melanocytes, pigmentation research), MC3R (immune modulation and energy balance research), MC4R (energy homeostasis and appetite regulation research), and MC5R (exocrine gland secretion research)
The effector T cells can be additionally partitioned into distinct subsets according to their cytokine profiles and genetic expression patterns
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