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retatrutide liver safety

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PA and OA (Millipore Sigma) were diluted stepwise in DMSO to 200 mM, and then to 100 mM in 10% fatty acid-free BSA (Millipore Sigma), followed by incubation at 40C for 90 min with gentle agitation

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After release of TML by lysosomal protein degradation, this compound is hydroxylated by TMLD, after which the resulting HTML is cleaved by a specific aldolase, which uses pyridoxal 5-phosphate (PLP) as a cofactor, into TMABA and glycine Substrate and Intermediate Compounds Trimethyllysine (TML) TML is the starting substrate for carnitine biosynthesis, derived from the methylation of lysine residues in proteins

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