At varying time points (0, 2, 5, 15, 30 min), purified Pt GSTF8 (8 M) and GSH (20 M) were added to an aliquot of the equilibration reaction and the resulting mixtures were incubated at 30 C for 30 min at 1200 rpm using a 3 mm shaking diameter before extraction with 2 vol of 75% methanol in H 2 O, 1 mM EDTA, 0.3% hydrochloric acid
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Journal of Nutrition for the Elderly
The peptide has been the subject of multiple in vitro and animal studies (and a smaller number of human observational studies) published in Bulletin of Experimental Biology and Medicine , Biogerontology , and other journals