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The analysis of semaglutide after synthesis was analyzed using ultra-high performance liquid chromatography-UV-mass spectrometry (UPLC-UV-MS) on a BEH130 C4 column with a mobile phase containing acetonitrile, water, and 0.1% trifluoroacetic acid [91]
& Yuan, G
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Western blot Equal amounts (50 g) of RINm5F, INS-1, and MIN6 cell lysate were analyzed by Western blotting with rabbit anti-AMPK monoclonal antibody#ab32047 (Epitomics Technology, Berlingame, CA), rabbit anti-phospho-AMPK(Thr172) monoclonal antibody #2531 (Cell Signaling Technology, Beverly, MA), rabbit anti-PARP polyclonal antibody#9542S, anti-cleaved caspase-3, phospho-CREB/CREB, PDX1, phospho Akt/Akt and GAPDH (Cell Signaling Technology, Beverly, MA), respectively
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