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Mutation of allosteric hot spot residues alter the allosteric communications In order to examine how the triangular allosteric network responds to the mutations of key residues, we have prepared three GDH mutants in silico using Chimera rotamers tools 29 GDH Ser441Leu , GDH Arg217Cys , and GDH Gly376Asp

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While the molecular ruthenium catalyst and the nanoreactors without carbon fluorinated moieties were cytotoxic in the low micromolar range in a 21% O 2 atmosphere, these compounds lost their therapeutic effect in a 1% O 2 atmosphere

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