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Cells were loaded with MitoTracker Red dye (Excitation/Emission: 579/599 nm) at 100 nM final concentration (37 C for 15 min) mounted in Live Cell Imaging Solution (Thermo Fisher), and viewed under a laser confocal microscope (LSM 510
Kutcher AM, LeBaron VT
10.1093/jb/mvab158 23
Unlike prior studies emphasizing single-organ effects, our analysis reveals that ferroptosis drives a self-reinforcing cycle: Hyperglycemia in DM triggers systemic iron overload, and the accumulation of iron in peripheral organs, combined with oxidative stress imbalance, causes organ damage (including pancreatic -cells), further exacerbating the progression of DM and forming a pathological feedback loop
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