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SDS-PAGE and western blotting Samples were loaded onto 10% SDS-polyacrylamide gels then transferred to 0.45 m PVDF membranes and blocked with 5% skimmed milk [or 5% bovine serum albumin (BSA) for anti-phospho antibodies] in TBS buffer supplemented with 0.1% Tween 20 (TBS-T)

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United States Department of Agriculture, Agricultural Research Service

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Actions of caffeine in the brain with special reference to factors that contribute to its widespread use

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