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At the same time, the Guidelines seek to minimise disruption to patients
10.1056/NEJMoa1401739 N Engl J Med
You simply run them concurrently
Reagent Caspase-3 (#9662, 1:1000), Cleaved caspase-3 (#9661, 1:1000), PARP (#9542, 1:1000), Bcl-xl (#2762, 1:1000), Bcl-2 (#2876, 1:1000), Bad (#9292, 1:1000), Bax (#2774, 1:1000), ATG-5 (#12994, 1:1000), ATG-7 (#8558, 1:1000), LC3A/B (#12741, 1:1000), Akt (#9272, 1:1000), p-Akt (Thr308) (#9275, 1:1000), p-mTOR (#2971, 1:1000), p-p70S6K (Thr389) (#9234, 1:1000) and -actin (#4970, 1:1000) antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA)
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