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cAMP accumulation assay Wild-type GLP-1R and GLP-1R mutants were cloned into the expression receptor pcDNA3.1/V5-His-TOPO vector (Invitrogen) at the HindIII and EcoRI sites by using the QuickChange site-directed mutagenesis and a flag-tag was inserted after native signal sequence (see primers in Supplementary Table 2)

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Some online peptide stores sell VIP-like products, but these are often marketed for research use only, and individuals should do their own research prior to purchasing

[16] The principal AOD9604 studies cited here did not establish a complete AOD9604-to-UCP1 pathway or demonstrate direct induction of UCP1 transcription, UCP1 activity, white-adipose browning, or functional thermogenesis

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