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In particular, since both Eb- and Ec-domains lacked N-glycosylation sites, the production of proIGF-1Eb and proIGF-1Ec was unaffected by inhibition of N-glycosylation (Figs 7A and S8C)

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For glutathione and glutamate quantification, cell viability analysis and oxidative stress assay, cells were sub-cultured in 12-well plates at a density of 4.2 10 5 cells/well