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Circulation 141 , 16621664 (2020)
Adipocyte tissue volume in bone marrow is increased with aging and in patients with osteoporosis
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Briefly, metabolites from 3 10 6 cells were extracted on ice with 0.8 ml pre-cold 80% methanol (80 C), and cell rupture was achieved by a Bullet Blender Storm 24 (Next Advance, Troy, NY) with metal beads, followed by five freeze-thaw out cycles
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