For the preparation of nuclear extracts, frozen liver pieces were homogenized in homogenization buffer (20 mM HEPES, 70 mM sucrose, 220 mM Mannitol, 2 mM EDTA, 0.5 mg/ml BSA, 0.1 mM PMSF, 1.0 mM DTT, pH 7.5)
RSL3, 0.5 M
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Its concentration decreases with age, which increases our skins susceptibility to oxidative stress and ageing
There is a growing body of evidence on the role of CAR on free radical scavenging, which has been also previously shown in in vitro and in vivo studies 36