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The recommended protocol rotates all three (PDRN, EGF, GHK-Cu) across sessions so each gets a fresh chamber and the underlying biology of each pathway has time to compound

Studies on topical delivery typically show effects concentrated in the epidermis and upper dermis

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Key biomarkers include glutathione levels, malondialdehyde (MDA), 8-isoprostane, and C-reactive protein to establish baseline oxidative burden