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The cell pellets were suspended in 800 L of lysis solution

In another paclitaxel-induced peripheral neuropathy (PIPN), 4-PSQ was observed to decrease paclitaxel-induced mechanical and thermal hypersensitivity, possibly related to modulation of oxidative stress in the spinal cord, hippocampus, and cerebral cortex of PIPN mice [163]

The higher dosage of 10mg per peptide (compared to the standard 5mg individual vials) provides researchers with sufficient material for extended experimental timelines without the need for frequent reconstitution, reducing contamination risk and improving protocol consistency

After 9 h of incubation, the 96-well plates were centrifuged and the supernatants were collected

The goal is a clear, stable solution at a known concentration (e.g., 1 mg/mL or 2 mg/mL) that allows accurate pipetting or syringe draws