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coli strain XL1-Blue and purified by affinity chromatography using an elution buffer containing 200 mM imidazole, 300 mM NaCl and 50 mM sodium phosphate, pH 8.0 (refs 11, 12, 18, 33, 53)

After many, many uses, the battery will simply wear out and be unable to hold on to any energy you put in during charging - most of it will be lost as heat