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If any serious side effects occur, consult a doctor immediately

Senescent but not young RBC, as obtained by density separation, are phagocytosed by macrophages, while phagocytosis of in vitro aged RBC requires opsonization with autologous IgG

Cells were then rinsed thrice using serum-free culture media to eliminate any extracellular DCFH-DA, and cells were imaged via fluorescent microscopy (Ex: 488 nm, Em: 522 nm) to assess intracellular ROS levels

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