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Senescent but not young RBC, as obtained by density separation, are phagocytosed by macrophages, while phagocytosis of in vitro aged RBC requires opsonization with autologous IgG

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Cells were then rinsed thrice using serum-free culture media to eliminate any extracellular DCFH-DA, and cells were imaged via fluorescent microscopy (Ex: 488 nm, Em: 522 nm) to assess intracellular ROS levels

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