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Proper peptide handling during preparation and storage helps reduce degradation processes such as hydrolysis, oxidation, and peptide aggregation that may affect analytical performance in experimental systems

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The diagnosis of 5-oxoprolinase deficiency is established by: Low activity of 5-oxoprolinase in nucleated cells such as leukocytes or cultured skin fibroblasts (5-oxoprolinase is not present in erythrocytes)

This can lead to improved energy levels and a reduction in the frequency and severity of fatigue-related symptoms