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Cells were seeded at a density of 5000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment

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Trulicity, which belongs to the same drug class as Ozempic (semaglutide) and Victoza (liraglutide), is FDA-approved to improve blood sugar levels in adults and children aged 10 years and older with Type 2 diabetes

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For testing on a first-time melanotan II subject, it is important to start with the lowest dose possible, before increasing as needed to achieve the desired outcome