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IGF-1 LR3 has been used extensively in this context precisely because its extended half-life allows sustained receptor activation in cell culture and in vivo models without the impractical dosing frequency that native IGF-1 would require

The siRNA sequences used were as follows: siRNA-AKT#1 (control): GCACCTTTATTGGCTACAA

DAPI was used to stain nuclei

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These cytokines can also cross the blood-brain barrier, driving the severe neuroinflammation responsible for the profound cognitive dysfunction, memory loss, and "brain fog" reported by so many patients